Welcome to the site for expansion microscopy protocols.
Here you can find relevant papers, protocols, materials lists, and advice on expansion microscopy (ExM), a new concept for imaging biological specimens with fine detail by physically making them bigger through a chemical process that preserves nanoscale isotropy. It enables super-resolution imaging on a conventional light microscope.
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References and Links
Chen, F.*, Tillberg, P.W.*, Boyden, E.S. (2015) Expansion Microscopy, Science 347(6221):543-548. (*, equal contribution) [Publisher link] [PDF]
Expansion microscopy (ExM) enables large 3-D volumes to be imaged with nanoscale precision, even on ordinary microscopes. It works by physically magnifying the sample itself. These protocols show you how to label your specimen with chemically anchorable dyes targeted via antibodies, synthesize swellable material evenly throughout specimens, and expand the resultant tissue-material hybrid. The net effect of the first version of expansion microscopy protocol boosts effective resolution of microscopes by ~4-4.5x. Please contact us with questions or suggestions.
Materials List, v1.3
This materials list contains an inventory of all the materials needed to carry out the ExM protocol.
ExM Cultured Cell Protocol, V1.4
A detailed description of how to expand cultured cells.
ExM Brain Slice Protocol, V1.4
A detailed description of how to expand brain slices.
DNA-Antibody Conjugation Protocol, V1.2
How to create ExM-ready secondary antibodies.